Chloroplastic ascorbate peroxidases targeted to stroma or thylakoid membrane: The chicken or egg dilemma

FEBS Letters

article

Gilberto Sachetto-Martins¹  Márcia Margis-Pinheiro²  Douglas Jardim-Messeder¹  Marcel Zamocky⁵ 

[1] Departamento de Genética, Universidade Federal do Rio de Janeiro;Departamento de Genética, Universidade Federal do Rio Grande do Sul;Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul;Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro;Laboratory of Phylogenomic Ecology, Institute of Molecular Biology, Slovak Academy of Sciences;Department of Chemistry, Institute of Biochemistry, University of Natural Resources and Life Sciences

关键词: alternative splicing;    antioxidant metabolism;    Archaeplastida;    ascorbate peroxidase;    chloroplasts;    gene duplication;    mitochondria;    neofunctionalization;    reactive oxygen species;   

DOI  :  10.1002/1873-3468.14438

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ascorbate peroxidases (APXs) are heme peroxidases that remove hydrogen peroxide in different subcellular compartments with concomitant ascorbate cycling. Here, we analysed and discussed phylogenetic and molecular features of the APX family. Ancient APX originated as a soluble stromal enzyme, and early during plant evolution, acquired both chloroplast-targeting and mitochondrion-targeting sequences and an alternative splicing mechanism whereby it could be expressed as a soluble or thylakoid membrane-bound enzyme. Later, independent duplication and neofunctionalization events in some angiosperm groups resulted in individual genes encoding stromal, thylakoidal and mitochondrial isoforms. These data reaffirm the complexity of plant antioxidant defenses that allow diverse plant species to acquire new means to adapt to changing environmental conditions.

【 授权许可】

Unknown