Growth-promoting effect on iron-sulfur proteins on axenic cultures of Entamoeba dispar

Parasite

and T. Takeuchi²  E. Hayakawa²  A. Haghighi¹  S. Kobayashi²  E. Imai²  S.A.M. Khalifa² 

[1] Department of Medical Parasitology and Mycology, School of Medicine Shaheed University of Medical Sciences,Eveen,Teheran 19395,Iran;Department of Tropical Medicine and Parasitology, School of Medicine, Keio University, Shinjuku-ku, Tokyo,Japan

关键词: Entamoeba dispar;    iron-sulphur protein;    chloroplasts;    hydrogenosomes;    mitochondria;    growth;   

Others  :  808733
DOI  :  doi:10.1051/parasite/2006131051

 received in 2005-06-14, accepted in 2005-11-04,  发布年份 2006

【 摘 要 】

A growth-promoting factor (GPF) that promotes the growth of Entamoeba dispar under axenic culture conditions was found in fractions of mitochondria (Mt), hydrogenosomes (Hg) and chloroplasts (Cp) obtained from cells of six different protozoan, mammalian and plant species. We were able to extract the GPF from the Cp-rich leaf cells of a plant (spiderwort: Commelina communis L.) in an acetone-soluble fraction as a complex of chlorophyll with low molecular weight proteins (molecular weight [MW] approximately 4,600). We also found that on treatment with 0.6 % complexes of 2-mercapthoethanol (2ME), complexes of chlorophyll-a with iron-sulphur (Fe-S) proteins (e.g., ferredoxins [Fd] from spinach and Clostridium pasteurianum) and noncomplex rubredoxin (Rd) from C. pasteurianum have a growth-promoting effect on E. dispar. These findings suggest that E. dispar may lack a sufficient quantity of some essential components of Fe-S proteins, such as Fe-S center.

【 授权许可】

   
© PRINCEPS Editions, Paris, 2006