Chemical Constituents of the Goat Margarine and Antibacterial Activity against Bacterial Pathogens in Sudan

Journal of Pure and Applied Microbiology

Rasha Khalid Abbas¹ 

[1] 1Depratment of Biochemistry, Faculty of Applied and Industrial Science University of Bahri, Sudan. 2Department of Chemistry, Faculty of Science and Arts in Mukhwa, University of Albaha, 65931, Al Bahah, Saudi Arabia.;

关键词: goat margarine;    gc-ms;    antibiotics;    pathogenic bacteria;    disc diffusion method.;   

DOI  :  10.22207/JPAM.13.1.23

来源: DOAJ

【 摘 要 】

The margarine constituents were examined by Gas Chromatography-Mass Spectrometry (GC-MS), it contained 41 compounds, fifteen of them were identified as the major compound, Hexadecanoic acid, methyl ester (22.39%), Methyl stearate (14.92%), methyl elaidate (13.80%), Methyl tetradecanoate (10.74%), Capric acid, methyl ester (8.34%) Lauric acid (4.52%) methyl octanoate (2.70%), linoleic acid methyl ester (2.57%), Methyl 11-octadecenoate (1.90%), methyl caproate (1.77%), Methyl pentadecanoate (1.72%), Methyl (8E,11E)-8,11-octadecadienoate(1.70%), Heptadecanoic acid, methyl ester, (1.46%),trans-13-Octadecenoic acid, methyl ester (Oleic acid) (1.20%), Methyl palmioleate (1.00%). The effect of goat margarine, against four different pathogenic bacteria Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, and Bacillus cereus, were carried out by using a disc diffusion technique, the highest antibacterial activity was detected against Salmonella typhimurium and the lowest one against Escherichia coli. The antibacterial activity of the antibiotics, (Ciprofloxacin, Tetracycline Ceftriaxone, Chloramphenicol and Gentamycin), were tested by the disc diffusion technique, and by measuring zones of inhibition, showsthattherewere differences, among all antibiotic thehighest activity of antibiotic against bacteria was due to the action of ciprofloxacin. Ceftriaxone and Tetracycline antibiotic give lowest activity. Among the bacteria the highest inhibition zone by antibiotic against Salmonella typhimurium, and the lowest one against Escherichia coli

【 授权许可】

Unknown