Validation of a LLME/GC-MS Methodology for Quantification of Volatile Compounds in Fermented Beverages

Molecules

Lucília Domingues¹  Zlatina Genisheva¹  Margarida Lemos¹  Eduardo Coelho¹  JoséM. Oliveira¹  Mar Vilanova² 

[1] CEB—Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal;Spanish National Research Council (MBG-CSIC), Salcedo, 36143 Pontevedra, Spain;

关键词: fermented beverages;    volatile compounds;    analytical method;    liquid-liquid microextraction;    gc-ms;   

DOI  :  10.3390/molecules25030621

来源: DOAJ

【 摘 要 】

Knowledge of composition of beverages volatile fraction is essential for understanding their sensory attributes. Analysis of volatile compounds predominantly resorts to gas chromatography coupled with mass spectrometry (GC-MS). Often a previous concentration step is required to quantify compounds found at low concentrations. This work presents a liquid-liquid microextraction method combined with GC-MS (LLME/GC-MS) for the analysis of compounds in fermented beverages and spirits. The method was validated for a set of compounds typically found in fermented beverages comprising alcohols, esters, volatile phenols, and monoterpenic alcohols. The key requirements for validity were observed, namely linearity, sensitivity in the studied range, accuracy, and precision within the required parameters. Robustness of the method was also evaluated with satisfactory results. Thus, the proposed LLME/GC-MS method may be a useful tool for the analysis of several fermented beverages, which is easily implementable in a laboratory equipped with a GC-MS.

【 授权许可】

Unknown