Molecular detection of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Tunisian dromedary camels (Camelus dromedarius)

Revue Marocaine des Sciences Agronomiques et Vétérinaires

卷:6

M.H. JEMLI¹  Z. AZAOUZI¹  L. MESSADI¹  I. ZAOUIA²  M.A. BEN HADJ KACEM³ 

[1] Department of Clinical Sciences, National School of Veterinary Medicine, Sidi Thabet, Manouba University, Tunisia;

[2] Directorate of Basic Health Care of Tunisia;

[3] Microbiology laboratory of the Charles Nicolle Hospital in Tunisia;

关键词: Middle East respiratory syndrome (MERS);    Camels;    Tunisia;    RT-PCR;    animal surveillance;    Coronavirus (CoVs);    molecular detection;   

DOI  :  

来源: DOAJ

【 摘 要 】

Middle East Respiratory Syndrome Coronavirus (MERS-CoV) was identified in humans in 2012. Dromedary camels were considered as the major reservoir of the virus throw several studies. Tunisian dromedary camels were implicated in a serological survey and a seropositivity reaching 100% was observed among some of them. Therefore, more investigations are necessary to look for the current infection situation. In this study, we aimed to detect the MERS-CoV virus in our camels by molecular technics. Blood and nasal swabs samples from 64 dromedary camels from 4 Tunisian provinces were collected during June 2014 and April 2015. All the animals tested negative by real time reverse transcriptase–polymerase chain reaction assay (RT-PCR). These findings could not confirm the absence of MERS-CoV active shading among the Tunisian camel population and more researches should be carried on to survey dromedary camels in Tunisia and its neighboring countries.

【 授权许可】

Unknown