Systemic transplantation of valproic acid primed human adipose stem cells on amelioration of motor deficits in animal model of cerebellar degeneration

مجله علمی دانشگاه علوم پزشکی کردستان

卷:23

Seyed Kamaladin Yazdanfar¹  Mohammad Amin Edalatmanesh² 

[1] Assistant Professor of Physiology, Department of Biology, College of Sciences, Shiraz Branch, Islamic Azad University, Shiraz, Iran;

[2] Ph.D. Candidate in Developmental Biology, Department of Biology, College of Sciences, Shiraz Branch, Islamic Azad University, Shiraz, Iran;

关键词: mesenchymal stem cell;    adipose tissue;    valproic acid;    cerebellum;    motor function;   

DOI  :  

来源: DOAJ

【 摘 要 】

Background and Aim: Cerebellar degeneration is characterized by disturbance in complexmotor functions, balance, muscular strength and motor learning. This study investigated theeffect of systemic transplantation of human adipose stem cells (hMSCs) primed with valproicacid (VPA) on amelioration of motor disorders in animal model of cerebellar degeneration.Material and Methods: After inducing unilateral lesion in rat cerebellum (Folia VI) byquinolinic acid, CM-DiI labeled hMSCs isolated from adipose tissue by liposuction, wereprimed with VPA for 48h and transplanted by way of the jugular vein, systemically. Then, weused cylinder, rotarod, hanging wire and beam balance test to assess motor functions in aperiod of 6 weeks after transplantation.Results: Six weeks after transplantation surviving hMSCs were detectable in the lesion site.The hMSC transplanted group showed markedly improved functional performance in motorlearning, locomotor asymmetry, dysmetria, and neuromuscular strength and balance, in thefirst 6 weeks after transplantation compared to the control group. On the other hand,transplantation of VPA primed hMSCs led to a better amelioration in motor functionscompared to non-primed hMSCs transplantation in the rats.Conclusion: According to the results of this study, cell therapy by means of VPA primedhMSCs can result in improved migration and homing capacity which can be regarded as apromising treatment for neurodegenerative disorders such as cerebellar diseases.

【 授权许可】

Unknown