Human Adipose-Derived Mesenchymal Stem Cells Are Resistant to HBV Infection during Differentiation into Hepatocytes in Vitro

International Journal of Molecular Sciences

Ying Wang³  Feng Wang³  Hongchang Zhao²  Xiaohe Zhang¹  Haiying Chen³ 

[1] Central Laboratory, Hepatobiliary Disease Hospital of Jilin Province, Changchun 130062, Jilin, China; E-Mail:;Biochemical Laborarory, Changchun Medical Emergency Center, Changchun 130062, Jilin, China; E-Mail:;Infection Department, First Hospital of Jilin University, Changchun 130021, Jilin, China; E-Mails:

关键词: hepatitis B;    mesenchymal stem cell;    adipose tissue;    hepatocyte-like cell;    glycogen;    albumin;   

DOI  :  10.3390/ijms15046096

来源: mdpi

【 摘 要 】

The therapeutic methods for chronic hepatitis B are limited. The shortage of organ donors and hepatitis B virus (HBV) reinfection obstruct the clinical application of orthotopic liver transplantation (OLT). In the present study, adipose-derived mesenchymal stem cells (AD-MSCs) and bone marrow-derived mesenchymal stem cells (BM-MSCs) were isolated from chronic hepatitis B patients and characterized for morphology, growth potency, surface phenotype and the differentiation potential. The results showed that both MSCs had adipogenic, osteogenic and neuron differentiation potential, and nearly all MSCs expressed CD105, CD44 and CD29. Compared with AD-MSCs, BM-MSCs of chronic hepatitis B patients proliferated defectively. In addition, the ability of AD-MSCs to differentiate into hepatocyte was evaluated and the susceptibility to HBV infection were assessed. AD-MSCs could differentiate into functional hepatocyte-like cells. These cells express the hepatic-specific markers and have glycogen production and albumin secretion function. AD-MSCs and hepatic differentiation AD-MSCs were not susceptible to infection by HBV in vitro. Compared with BM-MSCs, AD-MSCs may be alternative stem cells for chronic hepatitis B patients.

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