Effect of Matrigel on the Osteogenic Potential of Canine Adipose Tissue-Derived Mesenchymal Stem Cells

Journal of Veterinary Medical Science

Hak-Hyun RYU¹  Yongsun KIM¹  Oh-Kyeong KWEON¹  Heung-Myong WOO²  Sung-Su PARK¹  Wan Hee KIM¹  Byung-Jae KANG¹ 

[1] Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Daehak-dong, Gwanak-gu, Seoul 151–742, Korea;College of Veterinary Medicine and KNU Stem Cell Institute, Kangwon National University, Chuncheon 200–701, Korea

关键词: adipose tissue;    canine;    Matrigel;    mesenchymal stem cell;    osteogenesis;   

DOI  :  10.1292/jvms.11-0484

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(35)Cited-By(7)Adipose tissue-derived mesenchymal stem cells (Ad-MSCs) are a promising source of cells for bone tissue engineering. Matrigel is a basement membrane extract containing multiple extracellular components. This mixture may promote the osteogenic differentiation of MSCs and provide a more appropriate microenvironment for transplanted cells. Here, we investigated the effect of Matrigel on the osteogenic potential of Ad-MSCs. Canine Ad-MSCs were cultured in 2D and 3D matrices and implanted into subcutaneous pouches of dogs either with or without Matrigel. Culture mineralization, cell adhesion efficiency, cell proliferation, osteoid matrix production and alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase activities were quantified and compared. Ad-MSCs grown in 2D cultures with Matrigel showed higher levels of calcium deposition and ALP activity than those grown in the absence of Matrigel under osteogenic conditions. In 3D cultures, the cells cultivated with Matrigel showed greater attachment, proliferation and osteogenic differentiation than those grown without Matrigel. In vivo, Ad-MSCs implanted with Matrigel showed higher osteogenic potential than those without Matrigel. In conclusion, these data suggest that the use of Matrigel can increase the osteogenic potential of canine Ad-MSCs.

【 授权许可】

Unknown