Serological and molecular study of Hepatitis E virus in pediatric patients in Mexico

Annals of Hepatology

卷:19

Alicia Sotomayor-González¹  Francisco Arenas-Huertero²  Montserrat Elemi García-Hernández³  Blanca Itzel Taboada-Ramírez⁴  Rosa Elena Sarmiento-Silva⁴  María Elena Trujillo-Ortega⁴  Onofre Muñoz-Hernández⁵  Teresa Álvarez y Muñoz⁶  Tayde López-Santaella⁶  Alejandra Consuelo-Sánchez⁷  Sarbelio Moreno-Espinosa⁸  Mara Medeiros-Domingo⁹ 

[1] Corresponding author.;

[2] Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mor. Mexico;

[3] Departamento de Medicina y Zootecnia de Cerdos, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad de México, Mexico;

[4] Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad de México, Mexico;

[5] Dirección de Investigación, Hospital Infantil de México Federico Gómez, Ciudad de México, Mexico;

[6] Laboratorio de Investigación en Patología Experimental, Hospital Infantil de México Federico Gómez, Ciudad de México, Mexico;

[7] Servicio de Gastroenterología, Hospital Infantil de México Federico Gómez, Ciudad de México, Mexico;

[8] Servicio de Infectología, Hospital Infantil de México Federico Gómez, Ciudad de México, Mexico;

[9] Servicio de Nefrología, Hospital Infantil de México Federico Gómez, Ciudad de México, Mexico;

关键词: Virus;    Hepatitis;    Infectious disease;    Serology;    Pediatric patients;    HEV;   

DOI  :  

来源: DOAJ

【 摘 要 】

Introduction and objectives: Cases of viral hepatitis reported in Mexico are typically identified as hepatitis A, B and C. However, unspecified cases are reported annually. Hepatitis E virus (HEV) is an emergent agent that causes a self-limiting infection that can evolve to chronic in immunosuppressed individuals. In Mexico, HEV genotype 2 is considered endemic, though it's the prevalence is not well known. Therefore, the present study was designed to determine the prevalence of HEV among patients at the “Hospital Infantil de Mexico Federico Gomez”. Materials and methods: The study included 99 patients, anti-HEV antibody (IgG and IgM) were detected by indirect ELISA and viral genome was identified using RT-PCR technique. Two PCR products of positive cases were sequenced. Results: ELISA results were positive in 3% and 6%, for IgG and IgM respectively, 54.5% prevalence was found by PCR. Low lymphocyte count (p < 0.05) and malnutrition (p < 0.005) were significant factors for high PCR prevalence and could increase the possibility of infection. Two samples were sequenced and confirmed the presence of HEV genotype 3. Conclusions: This report reveals the incidence of HEV in pediatric patients in Mexico. Moreover, the identification of HEV genotype 3 in human samples suggests a potential zoonotic risk that requires further research.

【 授权许可】

Unknown