Antigenotoxic and antimutagenic effects of lignin derivative BP-C2 against dioxidine and cyclophosphamide in vivo in murine cells

Toxicology Reports

卷:9

Vladimir N. Anisimov¹  Andrey D. Durnev²  Elena A. Anisina³  Elena I. Fedoros³  Andrey V. Panchenko⁴  Zlata V. Chayka⁴  Sergey E. Pigarev⁴  Aliy K. Zhanataev⁵ 

[1] Correspondence to: Laboratory of molecular biology, FSBSI “Research Institute of Medical Primatology”, 177, Mira Str., s. Vesyoloe, Adlerskij r-n Sochi, Krasnodarskijkraj 354376, Russia.;

[2] FSBSI “Research Institute of Medical Primatology”, 177, Mira Str., s. Vesyoloe, Adlerskij r-n Sochi, Krasnodarskijkraj 354376, Russia;

[3] Nobel Ltd., of. 333, 271A, pr. Obukhovskoy oborony, Saint Petersburg 192012, Russia;

[4] Department of Carcinogenesis and Oncogerontology, FSBI “N.N. Petrov National Medical Research Center of Oncology” of Ministry of Health of the Russia, 68, Leningradskaya Str., Pesochny, Saint Petersburg 197758, Russia;

[5] FSBI “Zakusov Institute of Pharmacology”, 8, Baltijskaya Str., Moscow 125315, Russia;

关键词: Comet assay;    Chromosomal aberrations;    Lignin;    Polyphenols;    Genoprotective activity;    Antimutagenic activity;   

DOI  :  

来源: DOAJ

【 摘 要 】

The study investigated antigenotoxic and antimutagenic activity of novel lignin-derived polyphenolic composition (BP-C2) with ammonium molybdate towards cyclophosphamide and dioxidine in the bone marrow, blood and liver cells of BALB/c mice. BP-C2 was given to mice via gavage at 60, 80 and 120 mg/kg once 1 h before single intraperitoneal injection of a genotoxic agent. 1.5 h and 3 h after dioxidine or cyclophosphamide injection, respectively, cellular suspensions were obtained from mice and assessed with the comet test and cytogenetic analysis of bone marrow cells. It was observed that antigenotoxic activity of BP-C2 against DNA damage induced by dioxidine, a prooxidant genotoxic agent, in the bone marrow, liver and blood cells of mice in vivo was more pronounced at 60 and 80 mg/kg than at 120 mg/kg. When cyclophosphamide was used to induce a DNA damage, the genoprotective effect of BP-C2 was observed in bone marrow, liver and blood cells at 60 mg/kg dose but the effect was not significant at 80 mg/kg. When co-administered with 120 mg/kg BP-C2, cyclophosphamide induced a higher level of DNA damage in liver cells, but its genotoxic effect in bone marrow and blood cells was the same as when it was administered alone. When assessing the effect of BP-C2 on chromosomal aberrations induced by cyclophosphamide and dioxidine in bone marrow cells, it was revealed that all three tested doses of BP-C2 significantly decreased the number of cells with chromosome abnormalities. Thus, BP-C2 has a pronounced antimutagenic and genoprotective effects.

【 授权许可】

Unknown