Mass spectrometry and proteome analysis to identify SARS-CoV-2 protein from COVID-19 patient swab samples

STAR Protocols

卷:3

Mehar Un Nissa¹  Sanjeeva Srivastava¹  Arghya Banerjee¹  Avinash Singh¹  Medha Gayathri J Pai¹ 

[1] Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India;

关键词: Health Sciences;    Clinical Protocol;    Protein Biochemistry;    Proteomics;    Mass Spectrometry;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: With new emerging SARS-CoV-2 strains and their increased pathogenicity, diagnosis has become more challenging. Molecular diagnosis often involves the use of nasopharyngeal swabs and subsequent real-time PCR-based tests. Although this test is the gold standard, it has several limitations; therefore, more complementary assays are required. This protocol describes how to identify SARS-CoV-2 protein from patients' nasopharyngeal swab samples. We first introduce the approach of label-free quantitative proteomics. We then detail target verification by triple quadrupole mass spectrometry (MS)-based targeted proteomics.For complete details on the use and execution of this profile, please refer to Bankar et al. (2021).

【 授权许可】

Unknown