Comparative genome analysis of plant ascomycete fungal pathogens with different lifestyles reveals distinctive virulence strategies

BMC Genomics

Liping Hou¹  Ming Guo²  Quan Zou³  Jiacheng Yan⁴  Jie Wu⁴  Lei Xu⁵  Yansu Wang⁶ 

[1] Beidahuang Industry Group General Hospital, Harbin, China;Department of Agronomy and Horticulture, University of Nebraska, Lincoln, USA;Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, 610054, Chengdu, P. R. China;State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China;Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, 221116, Xuzhou, P. R. China;School of Electronic and Communication Engineering, Shenzhen Polytechnic, 518000, Shenzhen, P. R. China;School of Electronic and Communication Engineering, Shenzhen Polytechnic, 518000, Shenzhen, P. R. China;Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, 610054, Chengdu, P. R. China;

关键词: Plant fungal pathogens;    Carbohydrate-active enzymes;    Secondary metabolites;    Effector proteins;    Gene gain and loss;   

DOI  :  10.1186/s12864-021-08165-1

来源: Springer

【 摘 要 】

BackgroundPathogens have evolved diverse lifestyles and adopted pivotal new roles in both natural ecosystems and human environments. However, the molecular mechanisms underlying their adaptation to new lifestyles are obscure. Comparative genomics was adopted to determine distinct strategies of plant ascomycete fungal pathogens with different lifestyles and to elucidate their distinctive virulence strategies.ResultsWe found that plant ascomycete biotrophs exhibited lower gene gain and loss events and loss of CAZyme-encoding genes involved in plant cell wall degradation and biosynthesis gene clusters for the production of secondary metabolites in the genome. Comparison with the candidate effectome detected distinctive variations between plant biotrophic pathogens and other groups (including human, necrotrophic and hemibiotrophic pathogens). The results revealed the biotroph-specific and lifestyle-conserved candidate effector families. These data have been configured in web-based genome browser applications for public display (http://lab.malab.cn/soft/PFPG). This resource allows researchers to profile the genome, proteome, secretome and effectome of plant fungal pathogens.ConclusionsOur findings demonstrated different genome evolution strategies of plant fungal pathogens with different lifestyles and explored their lifestyle-conserved and specific candidate effectors. It will provide a new basis for discovering the novel effectors and their pathogenic mechanisms.

【 授权许可】

CC BY