The accumulation of two neolignan in the leaves, stems, and flower of red betel (Piper crocatum Ruiz & Pav.)

International Symposium on Bioinformatics, Chemometrics and Metabolomics

生物科学;化学

Hartini, Y.S.^1 ; Nugroho, L.^2

Faculty of Pharmacy, Sanata Dharma University, Yogyakarta, Indonesia^1

Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia^2

关键词: Chromatographic methods;    Gas chromatography-mass spectrometries (GC-MS);    Methanol extract;    Neolignan;    Neolignans;    Plant organs;    Secondary metabolites;    Shikimic acids;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/835/1/012017/pdf
DOI  :  10.1088/1742-6596/835/1/012017

学科分类：生物科学（综合）

来源: IOP

【 摘 要 】

Organ for the biosynthesis of secondary metabolite is not always a place for its biosynthesis, even the same compound was synthesized in the different organs in different plants. Neolignan is a secondary metabolite, known as an imunostimulant, synthesized through shikimic acid pathway with the important precursor is chorismic acid. The compound was known to be accumulated in the roots, stems and leaves of Piper regnellii with the concentration varies depending on the type of neolignan. It has been investigated the accumulation of two compound neolignans (Pc-1 and Pc-2) isolated from the methanol extract of red betel leaf (Piper crocatum Ruiz & Pav.) in the leaves, stems, and flowers of red betel. Chromatographic methods used was Gas Chromatography-Mass Spectrometry (GC-MS). Chromatogram of GC-MS showed that the Pc-1 with purity of 100%, m/z 460.3 could be detected at the minute of 29.986, while the Pc-2 with purity of 96.681%, m/z 418.3 was detected at the minute of 29.495. The research was then continued to investigate the existence and accumulation of both compounds in leaves, stems, and flowers of red betel. The GC-MS chromatogram shows that Pc-1 and Pc-2 could be detected in the leaves, stem and flower with various concentration among plant organs. Moreover, leaves contained the highest concentration of Pc-1 and Pc-2 compared to other plant organs.