Development of assay method by HPLC-DAD for the quantitative determination of chromones in Saposhnikovia divaricata radices and its validation

4th School-Seminar of Young Russian Scientists "Problems of Sustainable Regional Development", dedicated to the 70th anniversary of Academician of the Russian Academy of Sciences Arnold Kirillovich Tulokhonov

自然科学（总论）

Urbagarova, B.M.^1^2 ; Taraskin, V.V.^2 ; Shul'Ts, E.E.^3 ; Radnaeva, L.D.^1^2

Banzarov Buryat State University, Ulan-Ude

670000, Russia^1

Baikal Institute of Nature Management SB RAS, Ulan-Ude

670047, Russia^2

N N Vorozhtsov Novosibirsk Institute of Organic Chemistry, SB RAS, Novosibirsk

630090, Russia^3

关键词: Assay methods;    Chromatographic methods;    Chromones;    High performance liquid chromatography with diode array detection;    HPLC-DAD;    Optimal conditions;    Quantitative determinations;    Ultrasonic extraction;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/320/1/012056/pdf
DOI  :  10.1088/1755-1315/320/1/012056

学科分类：自然科学（综合）

来源: IOP

【 摘 要 】

Assay method using high performance liquid chromatography with diode array detection was developed and validated for the determination of chromones: prim-Oglucosylcimifugin, cimifugin, 4'-O-β-D-glucosyl-5-O-methylisamminol. Chromatographic method for the determination of chromones was validated for specificity, linearity, accuracy, repeatability and intermediate precision. The optimal conditions for the extraction of chromones from the Saposhnikoviae divaricatae radices were selected. It was found that these substances are better extracted with 50% ethanol using ultrasonic extraction at room temperature. The quantity of chromones in Saposhnikoviae Radix of the flora of Buryatia was also determined.