Menthol from the stem and leaf in-vitro Mentha piperita Linn.

2nd International Conference on Natural Resources and Life Sciences

生态环境科学

Wijaya, B.K.^1 ; Hardjo, P.H.^1 ; Emantoko, S.^1

Faculty of Biotechnology, University of Surabaya, Raya Kalirungkut Road, Surabaya, East Java

60292, Indonesia^1

关键词: Growth medium;    Mentha piperita;    menthol;    Morphological observations;    Plant tissue culture;    Secondary metabolites;    Shoot multiplication;    Steam distillation;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/293/1/012009/pdf
DOI  :  10.1088/1755-1315/293/1/012009

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

The need for menthol in Indonesia is increasing annually, but it is not followed by increases the availability in the country, so the number of imports is increasing each year. Therefore, in this research conducted a study using plant tissue culture techniques to produce secondary metabolite especially menthol using shoot multiplication. A single node of Mentha piperita L. was inoculated on three kinds of growth medium, which are MS + 0.1 mg L-1 NAA + 0.1 mg L-1 BA, MS + 0.5 mg L-1 BA, and MS + 2 mg L-1 BA. Based on fresh weight and morphological observation, MS + 0.1 mg L-1 NAA + 0.1 mg L-1 BA was the best growth medium. Based on the previous experiment, the study was aiming to continue observing the concentration of menthol found in M. piperita shoot. Stems and leaves of M. piperita were dried in room temperature, the dried stems and leaves were extracted using steam distillation method with ratio 0.3 g of dried stems and leaves in 25 mL aquadest. The crude extract was analyzed by gas chromatography (HP 6890) using INNOWAX 19095N-123 column. The results showed that 2 wk was the best amount of time to obtain the highest concentration of menthol specifically (1 218.5 ± 47.1) mg L-1 (yield = 9.748 %).