Modifications of proteoglycans secreted into the growth medium by young and senescent human skin fibroblasts

FEBS Letters

Campagnari, Francesco¹  Albertini, Riccardo¹  Passi, Alberto¹  De Luca, Giancarlo¹ 

[1] Department of Biochemistry ‘A. Castellani’, II Faculty of Medicine and Surgery, University of Pavia, via Taramelli 3b, 27100 Pavia, Italy

关键词: Proteoglycan;    Glycosaminoglycan;    Fibroblast;    Growth medium;    Replicative senescence;   

DOI  :  10.1016/S0014-5793(97)00008-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The properties of proteoglycans (PGs) secreted into the growth medium by normal young and senescent human skin fibroblasts (HFs) were investigated. In both cases, the incorporation per cell of radioactive precursors into total PGs was similar. The polysaccharide chains of PGs from young and senescent HFs were mainly represented by galactosaminoglycuronans and showed a similar range of size distribution. However, galactosaminoglycuronans of PGs secreted by senescent HFs had a lower content of unsulphated disaccharides and a lower proportion of d-glucuronosyl residues. Moreover, senescent HFs released into the growth medium higher relative amounts of small PGs with chondroitin sulphate, dermatan sulphate chains, such as decorin.

【 授权许可】

Unknown