Determining if T cell antigens are naturally processed and presented on HLA class I molecules

BMC Immunology

Christian Hinrichs¹  Maxwell Lee²  Jay Friedman²  Sreenivasulu Gunti²  Ke Bai²  Clint T. Allen² 

[1]Rutgers Cancer Center at Rutgers, The State University of New Jersey, New Brunswick, NJ, USA

[2]Section on Translational Tumor Immunology, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Building 10, Room 7N240C, 20892, Bethesda, MD, USA

关键词: HLA restriction;    Antigen presenting cells;    Minimal epitope;    Electroporation;    T cell responses;   

DOI  :  10.1186/s12865-022-00478-4

来源: Springer

【 摘 要 】

BackgroundDetermining T cell responses to naturally processed and presented antigens is a critical immune correlate to determine efficacy of an investigational immunotherapeutic in clinical trials. In most cases, minimal epitopes and HLA restriction elements are unknown.ResultsHere, we detail the experimental use of ex vivo expanded autologous B cells as antigen presenting cells to overcome the limitation of unknown HLA restriction, and the use of electroporated full length mRNA encoding full length parental proteins to ensure that any observed T cell responses are specific for antigens that are naturally processed and presented. ConclusionsThis technique can serve as useful experimental approach to determine the induction or enhancement of specific responses to naturally processed and presented antigens on HLA class I molecules in peripheral blood or tumor infiltrating T cells.

【 授权许可】

CC BY