The efficiency of MALDI-TOF MS method in detecting Staphylococcus aureus isolated from raw milk and artisanal dairy foods

CyTA - Journal of Food

Birce Mercanoglu Taban¹  Yasemin Numanoglu Cevik² 

[1] Dairy Technology Department, Faculty of Agriculture, Veterinary and Agriculture Campus, Ankara University, Diskapi, Ankara, Turke;Microbiology and Reference Laboratory and Biological Products Department, General Directorate of Public Health, Ministry of Health, Ankara, Turke;

关键词: Staphylococcus aureus;    raw milk;    matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS);    phyloproteomic principal component analysis (PCA);    artisanal dairy foods;    Staphylococcus aureus;    leche cruda;    espectrometría de masas de desorción/ionización láser asistida por matriz (EM MALDI-TOF);    análisis de componentes principales (PCA) filoproteómico;    alimentos lácteos artesanales;   

DOI  :  10.1080/19476337.2021.1977392

来源: Taylor & Francis

【 摘 要 】

The present study aims to characterize twenty-seven Staphylococcus aureus isolates among a total of forty-four Staphylococci recovered from raw milk and traditional artisanal dairy foods (n = 285) in Central Anatolia and the Mediterranean Regions of Turkey, using MALDI-TOF MS-based phyloproteomic principal component analysis (PCA). The data shown by the spectra were analyzed, followed by composite correlation index (CCI), virtual gel, and cluster analysis for validation. The correlation between standard strain S. aureus 25,923 THL (SA-18) and all strains was significant since protein peak of m/z 5305 ± 2 Da was common in all strains, including SA-18. Twenty-six (9.12%) and fifteen (5.26%) out of all samples were found as contaminated by Staphylococci and S. aureus, respectively. The findings suggest that MALDI-TOF MS-based PCA is a promising fast method to categorize S. aureus in dairy foods, in need of continuous monitoring of foodborne pathogens for public health.

【 授权许可】

CC BY