Coix lachryma-jobi extract ameliorates inflammation and oxidative stress in a complete Freund’s adjuvant-induced rheumatoid arthritis model

Pharmaceutical Biology

Shicheng Xie¹  Wenfeng Zhang²  Rongyu Shi³  Chunfang Zhang⁴  Bangyi Tang⁵ 

[1] Department of Joint Surgery, Affiliated Hospital of Jining Medical University, Shandong, Chin;Department of Orthopaedic Surgery, Yankuang Group General Hospital, Zoucheng, China;Department of Orthopaedics, Tancheng Hospital of Traditional Chinese Medicine, Tancheng, China;Department of Traditional Chinese Medicine, Xiang’an Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China;Medical College of Xiamen University, Xiamen, China;

关键词: Joint destruction;    paw oedema;    phenolic compounds;    antioxidant;   

DOI  :  10.1080/13880209.2019.1687526

来源: Taylor & Francis

【 摘 要 】

Context: Adlay seed [Job’s tears, Coix lachryma-jobi L. var. ma-yuen Stapf (Poaceae)] is a Traditional Chinese Medicine, which has been investigated to treat inflammatory diseases and rheumatism.Objective: This study evaluates the ameliorative effects of adlay seed extract (ASE) in a complete Freund’s adjuvant (CFA)-induced rheumatoid arthritis (RA) rats.Materials and methods: The RA Sprague–Dawley rat model was induced and randomly divided into six groups with or without ASE treatment (50, 100 or 200 mg/kg). After 28 d administration, the symptoms, biochemical parameters and molecular mechanisms were investigated.Results: The values of paw oedema, PGE2 and MMP-3 decreased from 1.46 ± 0.04 to 0.66 ± 0.07 cm3, from 126.2 ± 11.48 to 79.71 ± 6.8 pg/mL and from 142.7 ± 8.36 to 86.51 ± 5.95 ng/mL, respectively; the values of body weight increased from 177.25 ± 5.94 to 205 ± 6.52 g in HASE group. In addition, treatment of ASE reduced the levels of pro-inflammatory cytokines (IL-1β, TNF-α, IL-6, MCP-1), and increased the activities of antioxidant enzyme (GSH-Px, SOD, and CAT). Furthermore, ASE could suppress the mRNA expression of COX-2 and CHI3L1 and improve the mRNA expression of CAT and GPx-1 in ankle tissues of RA rats.Discussion and conclusions: For the first time, our results indicated ASE exerts anti-RA effects via inhibiting pro-inflammatory factors and alleviating oxidative stress. Our finding sheds light on the research and development of anti-RA functional foods from adlay seed.

【 授权许可】

CC BY