Biosynthesis of artemisinin – revisited

Journal of plant interactions

article

Nishi Srivastava¹  Anand Akhila¹ 

[1] Department of Chemistry, Central Institute of Medicinal and Aromatic Plants

关键词: Artemisia annua;    artemisinin;    biosynthesis;    sesquiterpene;    13 C-NMR;    mevalonic acid pathway (MVA);    deoxyxylulose pyrophosphate pathway (DXP);   

DOI  :  10.1080/17429145.2011.570869

学科分类：纳米科学和纳米技术

来源: Taylor & Francis

【 摘 要 】

Artemisinin is a well-known antimalarial drug isolated from the Artemisia annua plant. The biosynthesis of this well-known molecule has been reinvestigated by using [1- 13 C]acetate, [2- 13 C]acetate, and [1,6- 13 C 2 ]glucose. The 13 C peak enrichment in artemisinin was observed in six and nine carbon atoms from [1- 13 C]acetate and [2- 13 C]acetate, respectively. The 13 C NMR spectra of 13 C-enriched artemisinin suggested that the mevalonic acid (MVA) pathway is the predominant route to biosynthesis of this sesquiterpene. On the other hand, the peak enrichment of five carbons of 13 C-artemisinin including carbon atoms originating from methyls of dimethylallyl group of geranyl pyrophosphate (GPP) and farnesyl pyrophosphate (FPP) was observed from [1,6- 13 C 2 ]glucose. This suggested that GPP which is supposed to be biosynthesized in plastids travels from plastids to cytosol through the plastidial wall and combines with isopentenyl pyrophosphate (IPP) to form the ( E , E )-FPP which finally cyclizes and oxidizes to artemisinin. In this way the DXP pathway also contributes to the biosynthesis of this sesquiterpene.

【 授权许可】

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