Cellulose synthesizing Complexes in Vascular Plants andProcaryotes

Brown, Richard M, Jr ; Saxena, Inder Mohan

关键词: CELLULOSE;    CRYSTALLIZATION;    DISTRIBUTION;    DNA;    GENES;    MOLECULAR STRUCTURE;    PROTEINS;    REPLICAS;    TOBACCO;    VIGNA;    X-RAY DIFFRACTION cellulose;    biosynthesis;    enzyme comples;    microfibril;    cyanobacteria;    vigna;    acs gene;    gossypium;   

DOI  :  10.2172/958293
RP-ID  :  DOE-ER15396
PID  :  OSTI ID: 958293
Others  :  TRN: US201002%%345

美国|英语

来源: SciTech Connect

【 摘 要 】

Continuing the work initiated under DE-FG03-94ER20145, the following major accomplishments were achieved under DE-FG02-03ER15396 from 2003-2007: (a) we purified the acsD gene product of the Acetobacter cellulose synthase operon as well as transferred the CesA cellulose gene from Gossypium into E. coli in an attempt to crystallize this protein for x-ray diffraction structural analysis; however, crystallization attempts proved unsuccessful; (b) the Acetobacter cellulose synthase operon was successfully incorporated into Synechococcus, a cyanobacterium2; (c) this operon in Synechococcus was functionally expressed; (d) we successfully immunolabeled Vigna cellulose and callose synthase components and mapped their distribution before and after wounding; (e) we developed a novel method to produce replicas of cellulose synthases in tobacco BY-2 cells, and we demonstrated the cytoplasmic domain of the rosette TC; (f) from the moss Physcomitrella, we isolated two full-length cDNA sequences of cellulose synthase (PpCesA1 and PpCesA2) and attempted to obtain full genomic DNA sequences; (g) we examined the detailed molecular structure of a new form of non-crystalline cellulose known as nematic ordered cellulose (=NOC)3.