Genetic Diversity of Plasmodium vivax in Clinical Isolates from Southern Thailand using PvMSP1 , PvMSP3 ( PvMSP3α , PvMSP3β ) Genes and Eight Microsatellite Markers

The journal of physiological sciences

article

Supinya Thanapongpichat¹  Thunchanok Khammanee²  Nongyao Sawangjaroen²  Hansuk Buncherd¹  Aung Win Tun³ 

[1] Faculty of Medical Technology, Prince of Songkla University;Department of Microbiology, Faculty of Science, Prince of Songkla University;Faculty of Graduate Studies, Mahidol University

关键词: Plasmodium vivax;    malaria;    genetic diversity;    antigenic marker;    microsatellite marker;   

DOI  :  10.3347/kjp.2019.57.5.469

来源: Springer

【 摘 要 】

Plasmodium vivax is usually considered morbidity in endemic areas of Asia, Central and South America, and some part of Africa. In Thailand, previous studies indicated the genetic diversity of P. vivax in malaria-endemic regions such as the western part of Thailand bordering with Myanmar. The objective of the study is to investigate the genetic diversity of P. vivax circulating in Southern Thailand by using 3 antigenic markers and 8 microsatellite markers. Dried blood spots were collected from Chumphon, Phang Nga, Ranong and, Surat Thani provinces of Thailand. By PCR, 3 distinct sizes of PvMSP3α, 2 sizes of PvMSP3β and 2 sizes of PvMSP1 F2 were detected based on the length of PCR products, respectively. PCR/RFLP analyses of these antigen genes revealed high levels of genetic diversity. The genotyping of 8 microsatellite loci showed high genetic diversity as indicated by high alleles per locus and high expected heterozygosity (HE). The genotyping markers also showed multiple-clones of infection. Mixed genotypes were detected in 4.8% of PvMSP3α, 29.1% in PvMSP3β and 55.3% of microsatellite markers. These results showed that there was high genetic diversity of P. vivax isolated from Southern Thailand, indicating that the genetic diversity of P. vivax in this region was comparable to those observed other areas of Thailand.

【 授权许可】

Unknown