Maize catalases localized in peroxisomes support the replication of maize chlorotic mottle virus

Phytopathology Research

Siyuan Wang¹  Juan Wang¹  Tao Zhou¹  Yiying Tian¹  Xi Sun¹  Zaifeng Fan¹  Zhiyuan Jiao¹  Siqi Li¹  Wendi Ma¹ 

[1] State Kay Laboratory of Agrobiotechnology and Key Laboratory of Pest Monitoring and Green Management-MARA, Department of Plant Pathology, China Agricultural University, 100193, Beijing, China;

关键词: Catalase;    Maize chlorotic mottle virus (MCMV);    Peroxisome;    Replication site;    Virus replication;   

DOI  :  10.1186/s42483-021-00094-x

来源: Springer

【 摘 要 】

Co-infection of maize chlorotic mottle virus (MCMV) with a virus in the Potyviridae family, such as sugarcane mosaic virus, usually leads to maize lethal necrosis (MLN). Over the past decade, MCMV/MLN has emerged in many countries/regions of the world and resulted in serious yield loss in maize production. Although partial functions of some MCMV-encoded proteins have been identified, the host factors related to MCMV replication are poorly understood. Here, we show that maize peroxisomes can form aggregated bodies in MCMV-infected leaf cells. The dsRNA binding-dependent fluorescence complementation assay indicated that the aggregated peroxisomes in maize served as the major replication site of MCMV. In addition, our results revealed that all the three maize catalases were present mostly in peroxisomes in the presence or absence of MCMV. Furthermore, we determined that inhibition of catalase activity or induction of reactive oxygen species (ROS) in maize protoplasts significantly reduced the accumulation of MCMV RNA. In summary, this research reveals the replication site of MCMV and an important role of maize catalases in supporting virus replication. Our results are conducive to understanding the pathogenesis of MCMV and identifying targets for resistance breeding or gene regulation strategies.

【 授权许可】

CC BY