Rise and fall of peroxisomes during Alzheimer´s disease: a pilot study in human brains

Acta Neuropathologica Communications

Research

Barbara Ahlemeyer¹  Eveline Baumgart-Vogt¹  Eugen Semikasev²  Till Acker³  Anne Schänzer³ 

[1] Division of Medical Cell Biology, Institute for Anatomy and Cell Biology, Justus-Liebig University, Aulweg 123, 35385, Giessen, Germany;Division of Medical Cell Biology, Institute for Anatomy and Cell Biology, Justus-Liebig University, Aulweg 123, 35385, Giessen, Germany;Department of Neurosurgery, University Hospital of Giessen, Klinikstr. 33, 35392, Giessen, Germany;Institute of Neuropathology, Justus-Liebig University, Arndtstr. 16, 35392, Giessen, Germany;

关键词: Catalase;    Hippocampus;    Neocortex;    Neurodegenerative disorder;    Peroxisome;    PEX14;    Pyramidal neurons;   

DOI  :  10.1186/s40478-023-01567-0

 received in 2023-02-25, accepted in 2023-04-10,  发布年份 2023

来源: Springer

【 摘 要 】

Peroxisomes are eukaryotic organelles that rapidly change in number depending on the metabolic requirement of distinct cell types and tissues. In the brain, these organelles are essential for neuronal migration and myelination during development and their dysfunction is associated with age-related neurodegenerative diseases. Except for one study analysing ABCD3-positive peroxisomes in neurons of the frontal neocortex of Alzheimer disease (AD) patients, no data on other brain regions or peroxisomal proteins are available. In the present morphometric study, we quantified peroxisomes labelled with PEX14, a metabolism-independent peroxisome marker, in 13 different brain areas of 8 patients each either with low, intermediate or high AD neuropathological changes compared to 10 control patients. Classification of patient samples was based on the official ABC score. During AD-stage progression, the peroxisome density decreased in the area entorhinalis, parietal/occipital neocortex and cerebellum, it increased and in later AD-stage patients decreased in the subiculum and hippocampal CA3 region, frontal neocortex and pontine gray and it remained unchanged in the gyrus dentatus, temporal neocortex, striatum and inferior olive. Moreover, we investigated the density of catalase-positive peroxisomes in a subset of patients (> 80 years), focussing on regions with significant alterations of PEX14-positive peroxisomes. In hippocampal neurons, only one third of all peroxisomes contained detectable levels of catalase exhibiting constant density at all AD stages. Whereas the density of all peroxisomes in neocortical neurons was only half of the one of the hippocampus, two thirds of them were catalase-positive exhibiting increased levels at higher ABC scores. In conclusion, we observed spatiotemporal differences in the response of peroxisomes to different stages of AD-associated pathologies.Graphical Abstract

【 授权许可】

CC BY   
© The Author(s) 2023

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