Antimicrobial and plant growth-promoting activities of bacterial endophytes isolated from Calotropis procera (Ait.) W.T. Aiton

Biocell

article

MUHAMMAD HAMAYUN¹  NASIR KHAN¹  MUHAMMAD NAUMAN KHAN²  MUHAMMAD QADIR¹  ANWAR HUSSAIN¹  AMJAD IQBAL³  SUMERA AFZAL KHAN⁴  KHUSHNOOD UR REHMAN⁵  IN-JUNG LEE⁶ 

[1] Plant-Microbe Interactions Lab, Department of Botany, Abdul Wali Khan University Mardan;Department of Botany, Bacha Khan University Charsadda;Department of Food Science and Technology, Abdul Wali Khan University Mardan;Centre of Biotechnology and Microbiology, University of Peshawar;Department of Botany, Islamia College University;Department of Applied Biosciences, Kyungpook National University

关键词: Endophytic bacteria;    Growth promotion;    Antimicrobial activity;    Plant-microbe interaction;   

DOI  :  10.32604/biocell.2021.013907

学科分类：仪器

来源: Biocell

【 摘 要 】

Bacterial endophytes are beneficial to their hosts as they can fix nitrogen in the soil and make it available to the host. Endophytic bacteria also secrete plant growth-promoting hormones to support their host plants under normal as well as stress conditions. The current study aimed to isolate endophytic bacteria from different parts of Calotropis procera , i.e., roots, stem and leaves of Calotropis procera (Ait.) W.T. Aiton. Plants were collected from the Lundkhwar, district Mardan. A total of 12 bacterial strains, i.e., six from roots, three from the stem and three from the leaves were isolated. The strains were screened for their growth-promoting activity in rice plants because rice shows a quick and easy response to the bioactive compounds present in the culture filtrate (CF) of the potent endophytic strains. The rice plants were cultivated in pots containing 30 mL of 0.8% w/v water-agar medium. The pots were placed in a growth chamber, operated at 28 ± 0.3°C for 14 h (day); and 25 ± 0.3°C for 10 h (night), at 70% relative-humidity. Among the isolated strains, R1, S1, S3, L1, R5 and R6 showed visible growth promotion in rice plants. The biochemical analysis revealed that the strains were able to produce indole acetic acid (IAA) and flavonoids in higher quantities. Moreover, the strains also produced bioactive compounds that inhibited the growth of Escherichia coli and Aspergillus flavus using the well diffusion method. From the results, it was concluded that these strains can secrete potent compounds that can promote the host plant growth and inhibit the growth of pathogenic microorganisms and, therefore, can be used as bio-fertilizer and bio-control agents.

【 授权许可】

CC BY