Growth Promotion and Phytopathogen Inhibition Potentials of Lemon Grass (Cymbopogon citratus) Endophytic Bacteria

Jordan Journal of Biological Sciences

Abdullahi B. Inuwa¹ 

关键词: Endophytic bacteria;    Lemon grass;    Fusarium oxysporum;    Growth promotion;    Biocontrol.;   

DOI  :  

学科分类：生物科学（综合）

来源: Hashemite University, Deanship of Research and Higher Studies

【 摘 要 】

Fresh and apparently healthy leaves and roots of lemon grass were collected and surface - sterilized using 70% (v/v) Ethanol, 3% (v/v) sodium hypochlorite solution and sterile distilled water. Isolation of endophytic bacteria was achieved using culture technique while, characterization was done based on morphological, biochemical and microscopic characteristics. Growth promotion potentials of some selected isolates were tested using tomato and millet seeds. Similarly, antagonistic potentials against Fusarium oxysporum were evaluated. A total of 16 endophytic bacteria were isolated and identified as Bacillus spp (3 isolates), Escherichia coli (1 isolate), Klebsiella pneumoniae (3 isolates), Micrococcus spp (3 isolates), Pseudomonas spp (1 isolate), Rhizobium spp (2 isolates) and Staphylococcus aureus (3 isolates). Growth promotion test showed that, only K. pneumoniae significantly improved (P < 0.05) the germination time, germination percentage, shoot length and fresh weight of tomato seeds. None of the bacteria showed evidence of improving any of the parameters of germination of millet seeds. All the endophytic bacteria significantly inhibited (P < 0.05) the growth of F. oxysporum. S. aureus yielded the largest (21.30 mm) while, Bacillus cereus yielded the smallest (17.2 mm) zone of inhibition. Moreover, all the isolates especially S. aureus significantly inhibited (P < 0.05) the growth of F. oxysporum. In conclusion, Lemon grass harbours a variety of endophytic bacteria some of which showed potentials of enhancing the emergence and development of tomato seedling, and also have antagonistic activity against F. oxysporum.

【 授权许可】

CC BY