First report of garlic virus E in Australia

Australasian Plant Disease Notes

article

Nurulita, Sari¹  Geering, Andrew D. W.¹  Crew, Kathleen S.²  Harper, Stephen²  Thomas, John E.¹ 

[1] Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland;Queensland Department of Agriculture and Forestry

关键词: Allexivirus;    Allium;    RT-PCR;    Sequencing;   

DOI  :  10.1007/s13314-020-00400-0

学科分类：农业科学（综合）

来源: Springer

【 摘 要 】

Garlic virus E (GarVE) is reported for the first time in Australia, from four accessions in a garlic germplasm collection planted at Gatton in South East Queensland. The presence of the virus was confirmed by reverse transcriptase PCR using GarVE-specific primers, followed by direct sequencing of the PCR product. The isolates had 99.1–99.7% nucleotide identity to each other, and 97.9–98.5% nucleotide sequence identity to the exemplar isolate for the species within the amplified region covering parts of the coat protein and nucleic acid binding protein genes. The distribution and economic impact of the virus in commercial plantings needs to be determined.

【 授权许可】

CC BY