期刊论文详细信息
BMC Infectious Diseases
Molecular characterization of influenza viruses collected from young children in Uberlandia, Brazil - from 2001 to 2010
Research Article
Hélio Lopes da Silveira1  Thelma Fátima de Mattos Silva Oliveira2  Jonny Yokosawa2  Divina Aparecida Oliveira Queiróz2  Fernando Couto Motta3  Marilda Mendonça Siqueira3 
[1] Faculdade de Medicina – UFU, Uberlândia, MG, Brazil;Laboratório de Virologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia (UFU), Uberlândia, MG, Brazil;Laboratório de Vírus Respiratórios, Instituto Oswaldo Cruz, Fiocruz, RJ, Brazil;
关键词: Influenza virus;    Children;    RT-PCR;    Sequencing;    Hemagglutinin;    Neuraminidase;   
DOI  :  10.1186/s12879-015-0817-z
 received in 2014-09-26, accepted in 2015-02-10,  发布年份 2015
来源: Springer
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【 摘 要 】

BackgroundInfluenza remains a major health problem due to the seasonal epidemics that occur every year caused by the emergence of new influenza virus strains. Hemagglutinin (HA) and neuraminidase (NA) glycoproteins are under selective pressure and subjected to frequent changes by antigenic drift. Therefore, our main objective was to investigate the influenza cases in Uberlândia city, Midwestern Brazil, in order to monitor the appearance of new viral strains, despite the availability of a prophylactic vaccine.MethodsNasopharyngeal samples were collected from 605 children less than five years of age presenting with acute respiratory disease and tested by immunofluorescence assay (IFA) for detection of adenovirus, respiratory syncytial virus, parainfluenza virus types 1, 2, and 3 and influenza virus types A and B. A reverse transcription-PCR (RT-PCR) for influenza viruses A and B was carried out to amplify partial segments of the HA and NA genes. The nucleotide sequences were analyzed and compared with sequences of the virus strains of the vaccine available in the same year of sample collection.ResultsForty samples (6.6%) were tested positive for influenza virus by IFA and RT-PCR, with 39 samples containing virus of type A and one of type B. By RT-PCR, the type A viruses were further characterized in subtypes H3N2, H1N2 and H1N1 (41.0%, 17.9%, and 2.6%, respectively). Deduced amino acid sequence analysis of the partial hemagglutinin sequence compared to sequences from vaccine strains, revealed that all strains found in Uberlândia had variations in the antigenic sites. The sequences of the receptor binding sites were preserved, although substitutions with similar amino acids were observed in few cases. The neuraminidase sequences did not show significant changes. All the H3 isolates detected in the 2001-2003 period had drifted from vaccine strain, unlike the isolates of the 2004-2007 period.ConclusionsThese results suggest that the seasonal influenza vaccine effectiveness could be reduced because of A H3N2 variants that circulated in 2001-2003 years. Thus, an early monitoring of variants circulating in the country or in a region may provide important information about the probable efficacy of the vaccine that will be administered in an influenza season.

【 授权许可】

CC BY   
© de Mattos Silva Oliveira et al.; licensee BioMed Central. 2015

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