Adipose-derived stem cells therapy effectively attenuates PM2.5-induced lung injury

Stem Cell Research & Therapy

Junling Gao¹  Zhongbing Lu¹  Yuanli Wang¹  Juntao Yuan¹  Wenjun Ding¹  Yingjie Chen²  Guangju Ji³  Qun Liu³  Huiwen Wang³ 

[1] College of Life Science, University of Chinese Academy of Sciences, 19A Yuquanlu, 100049, Beijing, China;Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, USA;Institute of Biophysics, Chinese Academy of Sciences, Datun Road 15, Chaoyang district, 100101, Beijing, China;

关键词: Adipose-derived stem cells;    PM2.5;    inflammation;    Pyroptosis;   

DOI  :  10.1186/s13287-021-02441-3

来源: Springer

【 摘 要 】

BackgroundThe adverse health effects of fine particulate matter (PM2.5) exposure are associated with marked inflammatory responses. Adipose-derived stem cells (ADSCs) have immunosuppressive effects, and ADSC transplantation could attenuate pulmonary fibrosis in different animal disease models. However, whether ADSCs affect PM2.5-induced lung injury has not been investigated.MethodC57BL/6 mice were exposed to PM2.5 every other day via intratracheal instillation for 4 weeks. After that, the mice received tail vein injections of ADSCs every 2 weeks.ResultsADSC transplantation significantly attenuated systemic and pulmonary inflammation, cardiac dysfunction, fibrosis, and cell death in PM2.5-exposed mice. RNA-sequencing results and bioinformatic analysis suggested that the downregulated differentially expressed genes (DEGs) were mainly enriched in inflammatory and immune pathways. Moreover, ADSC transplantation attenuated PM2.5-induced cell apoptosis and pyroptosis in the lungs and hearts.ConclusionADSCs protect against PM2.5-induced adverse health effects through attenuating pulmonary inflammation and cell death. Our findings suggest that ADSC transplantation may be a potential therapeutic approach for severe air pollution-associated diseases.

【 授权许可】

CC BY