A Novel Herbal Extract Blend Product Prevents Particulate Matters-Induced Inflammation by Improving Gut Microbiota and Maintaining the Integrity of the Intestinal Barrier

Nutrients

Mark Bartlett¹  Lu Deng²  Chang Liu²  Lilan Jin²  Hai Wang²  Yixiao Pan²  Qian Chen³  Yiping Ren⁴  Jihong Lu⁴  Xiaokui Guo⁵ 

[1] Center for Anti-Aging Research, Nu Skin Enterprises, Provo, UT 84601, USA;Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China;Institute of Intestinal Diseases, Shanghai Tenth People’s Hospital Affiliated to Tongji University, Shanghai 200072, China;Nu Skin (China) Daily-Use & Health Products Co., Ltd., Center of Anti-Aging Research, Nu Skin Enterprises, Shanghai 201401, China;School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China;

关键词: PM2.5;    inflammation;    gut microbiota;    herbal extract blend;   

DOI  :  10.3390/nu14102010

来源: DOAJ

【 摘 要 】

Air pollutants of PM2.5 can alter the composition of gut microbiota and lead to inflammation in the lung and gastrointestinal tract. The aim of this study was to evaluate the protective effect of a novel herbal extract blend, FC, composed of Lonicera japonica extract, Momordica grosvenori extract, and broccoli seed extract, on PM2.5-induced inflammation in the respiratory and intestinal tract. A549 cells and THP-1 cells, as well as C57BL/6 mice, were stimulated with PM2.5 to establish in vitro and in vivo exposure models. The models were treated with or without FC. The expression of inflammatory cytokines and tight junction proteins were studied. Proteomic analysis was performed to elucidate mechanisms. Mouse feces were collected for gut microbiota analysis. FC was shown to modulate the upregulation of pro-inflammatory cytokines mRNA expression in A549 and THP-1 cells and downregulated tight junction proteins mRNA expression in A549 cells due to PM2.5 stimulation. In animal models, the decreased expression of the anti-inflammatory factor il-10, tight junction protein ZO-1, and the elevated expression of COX-2 induced by PM2.5 were improved by FC intervention, which may be associated with zo-1 and cox-2 signaling pathways. In addition, FC was shown to improve the gut microbiota by increasing the abundance of beneficial bacteria.

【 授权许可】

Unknown