The mechanisms of colorectal cancer cell mesenchymal-epithelial transition induced by hepatocyte exosome-derived miR-203a-3p

BMC Cancer

Yongliang Huang¹  Wei Lai²  Qiusheng Lan²  Yujie Zeng²  Zhonghua Chu²  Ziqiang Chu²  Heyang Xu²  Pengwei Su²  Yang Zhang³ 

[1] Department of General Surgery, Foshan Maternal and Child Health Hospital, Southern Medical University, Foshan, China;Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Department of Gastrointestinal Surgery Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China;Guangzhou Blood Center, Guangzhou, Guangdong Province, China;

关键词: miR-203a-3p;    Exosome;    Mesenchymal-to-epithelial transition;    Colorectal cancer;   

DOI  :  10.1186/s12885-021-08419-x

来源: Springer

【 摘 要 】

BackgroundLiver metastasis is the most common cause of death in patients with colorectal cancer (CRC). Phosphatase of regenerating liver-3 induces CRC metastasis by epithelial-to-mesenchymal transition, which promotes CRC cell liver metastasis. Mesenchymal-to-epithelial transition (MET), the opposite of epithelial-to-mesenchymal transition, has been proposed as a mechanism for the establishment of metastatic neoplasms. However, the molecular mechanism of MET remains unclear.MethodsUsing Immunohistochemistry, western blotting, invasion assays, real-time quantitative PCR, chromatin immunoprecipitation, luciferase reporter assays, human miRNA arrays, and xenograft mouse model, we determined the role of hepatocyte exosome-derived miR-203a-3p in CRC MET.ResultsIn our study, we found that miR-203a-3p derived from hepatocyte exosomes increased colorectal cancer cells E-cadherin expression, inhibited Src expression, and reduced activity. In this way miR-203a-3p induced the decreased invasion rate of CRC cells.CoclusionMiR-203a-3p derived from hepatocyte exosomes plays an important role of CRC cells to colonize in liver.

【 授权许可】

CC BY