Immortalization of neuronal progenitors using SV40 large T antigen and differentiation towards dopaminergic neurons

Journal of Cellular and Molecular Medicine

A. Alwin Prem Anand²  S. Gowri Sankar¹ 

[1] Department of Biotechnology, Anna University, Tiruchirappalli, India;Institute of Anatomy, University of Tübingen, Tübingen, Germany

关键词: SV40 large T antigen;    neuronal progenitors;    dopaminergic neurons;    Parkinson's disease;    immortalized cell lines;    stem cells;    transplantation.;   

DOI  :  10.1111/j.1582-4934.2012.01607.x

来源: Wiley

【 摘 要 】

Abstract

Transplantation is common in clinical practice where there is availability of the tissue and organ. In the case of neurodegenerative disease such as Parkinson's disease (PD), transplantation is not possible as a result of the non-availability of tissue or organ and therefore, cell therapy is an innovation in clinical practice. However, the availability of neuronal cells for transplantation is very limited. Alternatively, immortalized neuronal progenitors could be used in treating PD. The neuronal progenitor cells can be differentiated into dopaminergic phenotype. Here in this article, the current understanding of the molecular mechanisms involved in the differentiation of dopaminergic phenotype from the neuronal progenitors immortalized with SV40 LT antigen is discussed. In addition, the methods of generating dopaminergic neurons from progenitor cells and the factors that govern their differentiation are elaborated. Recent advances in cell-therapy based transplantation in PD patients and future prospects are discussed.

【 授权许可】

Unknown   
© 2012 The Authors Journal of Cellular and Molecular Medicine © 2012 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd