Prevalence of Mycobacterium avium subsp. paratuberculosis Fecal Shedding in Alpacas Presented to Veterinary Hospitals in the United States

Journal of Veterinary Internal Medicine

M.-E. Fecteau²  D. Bedenice⁴  C.K. Cebra³  T.L. Pinn¹  S.C. McAdams²  T.L. Fyock²  R.H. Whitlock² 

[1] Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY;Department of Clinical Studies-New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, PA;Department of Clinical Sciences, Oregon State University College of Veterinary Medicine, Corvallis, OR;Department of Clinical Sciences, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA

关键词: Camelid;    Detection;    Infectious diseases;   

DOI  :  10.1111/jvim.12125

来源: Wiley

【 摘 要 】

Abstract

Background

The prevalence of Johne's disease in alpacas in the United States is unknown. The limits of polymerase chain reaction (PCR) detection of Mycobacterium avium subsp. paratuberculosis (MAP) in alpaca feces have not been determined.

Objectives

To evaluate the use of PCR for MAP detection in alpaca feces; and to estimate the prevalence of MAP fecal shedding in alpacas presented to veterinary teaching hospitals.

Animals

Alpacas presenting to 4 US veterinary teaching hospitals from November 2009 to February 2011.

Methods

Prospective study. Ten dilutions of a wild MAP strain were added to negative alpaca feces and processed for MAP detection by means of a commercial real-time PCR (RT-PCR) assay, and cultured on Herrold's Egg Yolk Medium (HEYM) and liquid broth. The limits of detection for each method were determined. Fecal samples from alpacas admitted to the veterinary teaching hospitals during the study period were evaluated for MAP via PCR and HEYM.

Results

The lowest MAP dilution detectable via PCR was 243 MAP colony-forming units (CFU)/g of feces, at which concentration MAP growth was detectable on HEYM. Ten (6%; 95% confidence interval: 3–9%) of the 180 fecal samples collected were positive on PCR.

Conclusions and Clinical Importance

Polymerase chain reaction can provide an accurate and rapid detection of MAP fecal shedding in alpacas; and the prevalence of MAP fecal shedding in hospitalized alpacas in 4 US veterinary teaching hospitals was 6%.

【 授权许可】

Unknown   
Copyright © 2013 by the American College of Veterinary Internal Medicine