Preparation, characterisation, drug loading and release properties of a novel KIT-6/poly(AA-EGDMA) nanocomposite

Micro & nano letters

article

Anahita Shafiei¹  S. Yahya¹  Sh. Beheshtiha¹  Majid M. Heravi¹  Roozbeh Javad Kalbasi² 

[1] Department of Chemistry, Alzahra University;Faculty of Chemistry, Kharazmi University

关键词: drug delivery systems;    nanocomposites;    filled polymers;    mesoporous materials;    silicon compounds;    nanomedicine;    biomedical materials;    nanofabrication;    polymerisation;    sol-gel processing;    Fourier transform infrared spectra;    X-ray diffraction;    thermal analysis;    scanning electron microscopy;    ultraviolet spectra;    visible spectra;    adsorption;    SiO2;    thermal gravimetric analysis;    simulated body fluid;    drug adsorption;    time-loading;    polymer;    ultraviolet-visible spectroscopy;    scanning electron microscopy;    Brunauer-Emmett-Teller specific surface areas;    X-ray powder diffraction;    Fourier transform infrared spectroscopy;    ibuprofen system;    mesostructure;    KIT-6 pores;    in situ method;    polymerisation;    sol-gel method;    three-dimensional cubic symmetric structure;    KIT-6-poly(acrylic acid-ethylene glycol dimethacrylate) nanocomposite;    KIT-6-poly(AA-EGDMA) nanocomposite;    drug release properties;    drug loading properties;   

DOI  :  10.1049/mnl.2017.0451

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

Background: Combretum micranthum G. Don. (Combretaceae) is an ethnomedicinally valuable, undomesticated and indigenous shrub of West Africa. However, its anxiolytic potential have not been reported despite its ethanolic extract being used ethnomedicinally in the management of anxiety disorders. Aim: To determine the acute toxicity effect and assess the behavioural effects and anxiolytic potential of C. micranthum G. Don. leaves in mice. Settings: This study is an experimental design to evaluate the ethnomedicinal claim of Combretum micranthum G. Don using animal models of anxiety. Methods: Fifty-six male and female mice, ranging in weight between 20 g and 30 g were randomly distributed into three main groups. The first group of mice ( n = 6) was assigned for toxicity assessment (LD 50 ) study using the guideline of Organization for Economic Cooperation and Development (OECD). The second group of mice for behavioural study ( n = 25) was further divided into five sub-groups. Sub-groups I, II and III were orally administered 500 mg/kg, 1000 mg/kg, 2000 mg/kg of ethanolic extract of C. micranthum ( Cm EE), respectively, whilst IV and V were intraperitoneally administered 1 mg/kg diazepam and normal saline 0.5 mL, respectively. They were thereafter evaluated for novelty-induced behaviours: locomotion, rearing and grooming using Open Field Test (OFT). The third group of mice ( n = 25) was treated similar to the pattern used in behavioural study and evaluated for anxiolytic activity of Cm EE using elevated plus maze (EPM) model. Data were expressed as mean ± standard error of mean (S.E.M) and analysed using Student’s- t test, and one-way analysis of variance (ANOVA) followed by Student–Newman– Keuls (SNK) test with values of p < 0.05 considered significant. Results: The percentage yield of ethanolic leaf extract of C. micranthum was 14.28% weight/weight (w/w). Combretum micranthum showed no toxicity when administered orally to mice (LD 50 ≥ 2000 mg/kg). Groups administered 500, 1000 and 2000 mg/kg of Cm EE exhibited decreased locomotion ( p < 0.05) when compared with saline group. There was significant decrease in rearing at 2000 mg/kg but increase in grooming in mice administered 2000 mg/kg of Cm EE was recorded. The groups administered 500, 1000 and 2000 mg/kg of Cm EE showed increased percentage time spent in the open arm in a dose-dependent pattern (33.3%, 41.6% and 55.4%, respectively) when compared with the saline group. There were significant dose-dependent decreases in the indices of open arm avoidance at 1000 (48.9) and 2000 mg/kg (41.4) of Cm EE. Conclusion: Combretum micranthum is non-toxic and preliminary data indicated that it possesses anxiolytic potential. However, it is recommended that further assays using other specific models of anxiety to determine its probable mechanism(s) of action should be explored.

【 授权许可】

CC BY|CC BY-ND|CC BY-NC|CC BY-NC-ND