Development of in-flow label-free single molecule sensors using planar solid-state nanopore integrated microfluidic devices

Micro & nano letters

article

Fatma D. Güzel¹  Benjamin Miles² 

[1] Biomedical Engineering, Ankara Yildirim Beyazit University;Department of Chemistry, Imperial College London, South Kensington Campus

关键词: biological techniques;    microfluidics;    nanosensors;    biosensors;    DNA;    electrochemical sensors;    silicon;    nanoporous materials;    flow sensors;    flow measurement;    microsensors;    silicon compounds;    elemental semiconductors;    in-flow label-free single molecule sensors;    label-free single molecule detection capability;    electrochemical sensing;    in-flow label-free electrochemical detection;    hydrodynamic flow;    planar solid-state nanopore integrated microfluidic devices;    DNA molecule sequencing;    solid-state nanopore biosensors;    graphene;    biomolecule sensing;    Si3N4-Si;   

DOI  :  10.1049/mnl.2018.5206

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

Nanopore biosensors have attracted attention due to their label-free single molecule detection capability. To date, different materials and applications have been shown in the field, varying from Si 3 N 4 to graphene and biomolecule sensing to DNA sequencing. Classical nanopore devices are composed of Si 3 N 4 material supported on a Si wafer and the detection is largely based on electrochemical sensing using chambers of ml volumes on both sides of the nanopore device. In this study, in-flow label-free electrochemical detection of DNA molecules at single molecule level is shown using a classical Si 3 N 4 nanopore device integrated into a microfluidic device. The layout of the device given here set the basics for future works and discussions regarding future microfluidic integrated solid-state nanopores and the behaviour of the molecule under the influence of hydrodynamic flow.

【 授权许可】

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