Micro & nano letters | |
Development of in-flow label-free single molecule sensors using planar solid-state nanopore integrated microfluidic devices | |
article | |
Fatma D. Güzel1  Benjamin Miles2  | |
[1] Biomedical Engineering, Ankara Yildirim Beyazit University;Department of Chemistry, Imperial College London, South Kensington Campus | |
关键词: biological techniques; microfluidics; nanosensors; biosensors; DNA; electrochemical sensors; silicon; nanoporous materials; flow sensors; flow measurement; microsensors; silicon compounds; elemental semiconductors; in-flow label-free single molecule sensors; label-free single molecule detection capability; electrochemical sensing; in-flow label-free electrochemical detection; hydrodynamic flow; planar solid-state nanopore integrated microfluidic devices; DNA molecule sequencing; solid-state nanopore biosensors; graphene; biomolecule sensing; Si3N4-Si; | |
DOI : 10.1049/mnl.2018.5206 | |
学科分类:计算机科学(综合) | |
来源: Wiley | |
【 摘 要 】
Nanopore biosensors have attracted attention due to their label-free single molecule detection capability. To date, different materials and applications have been shown in the field, varying from Si 3 N 4 to graphene and biomolecule sensing to DNA sequencing. Classical nanopore devices are composed of Si 3 N 4 material supported on a Si wafer and the detection is largely based on electrochemical sensing using chambers of ml volumes on both sides of the nanopore device. In this study, in-flow label-free electrochemical detection of DNA molecules at single molecule level is shown using a classical Si 3 N 4 nanopore device integrated into a microfluidic device. The layout of the device given here set the basics for future works and discussions regarding future microfluidic integrated solid-state nanopores and the behaviour of the molecule under the influence of hydrodynamic flow.
【 授权许可】
CC BY|CC BY-ND|CC BY-NC|CC BY-NC-ND
【 预 览 】
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RO202107100003039ZK.pdf | 343KB | download |