Microfluidics without channels: highly-flexible synthesis on a digital-microfluidic chip for production of diverse PET tracers

Van Dam, Robert Michael¹ 

[1] Univ. of California, Los Angeles, CA (United States)

关键词: positron emission tomography;    PET;    microfluidics;    electrowetting on dielectric;    EWOD;    automation;    miniaturization;    radiochemistry;    radiosynthesis;    radiosynthe;   

DOI  :  10.2172/1170744
RP-ID  :  DOE-UCLA--SC0005056
PID  :  OSTI ID: 1170744

美国|英语

来源: SciTech Connect

【 摘 要 】

Positron emission tomography (PET) imaging is used for fundamental studies of living biological organisms and microbial ecosystems in applications ranging from biofuel production to environmental remediation to the study, diagnosis, and treatment monitoring of human disease. Routine access to PET imaging, to monitor biochemical reactions in living organisms in real time, could accelerate a broad range of research programs of interest to DOE. Using PET requires access to short-lived radioactive-labeled compounds that specifically probe the desired living processes. The overall aims of this project were to develop a miniature liquid-handling technology platform (called ???microfluidics???) that increases the availability of diverse PET probes by reducing the cost and complexity of their production. Based on preliminary experiments showing that microfluidic chips can synthesis such compounds, we aimed to advance this technology to improve its robustness, increase its flexibility for a broad range of probes, and increase its user-friendliness. Through the research activities of this project, numerous advances were made; Tools were developed to enable the visualization of radioactive materials within microfluidic chips; Fundamental advances were made in the microfluidic chip architecture and fabrication process to increase its robustness and reliability; The microfluidic chip technology was shown to produce useful quantities of an example PET probes, and methods to further increase the output were successfully pursued; A ???universal??? chip was developed that could produce multiple types of PET probes, enabling the possibility of ???on demand??? synthesis of different probes; and Operation of the chip was automated to ensure minimal radiation exposure to the operator Based on the demonstrations of promising technical feasibility and performance, the microfluidic chip technology is currently being commercialized. It is anticipated that costs of microfluidic chips can be dramatically reduced in the future by leveraging economies of scale in the microelectronics industry, leading to low cost production of PET probes.