A mouse model for the study of transplanted bone marrow mesenchymal stem cell survival and proliferation in lumbar spinal fusion

European spine journal

article

Ioan A. Lina¹  Wataru Ishida²  Jason A. Liauw²  Sheng-fu L. Lo²  Benjamin D. Elder³  Alexander Perdomo-Pantoja²  Debebe Theodros²  Timothy F. Witham²  Christina Holmes² 

[1] Department of Otolaryngology, The Johns Hopkins University School of Medicine;Department of Neurosurgery, The Johns Hopkins University School of Medicine;Department of Neurological Surgery, Mayo Clinic School of Medicine

关键词: Spinal fusion;    Mouse model;    Bone marrow;    Luciferase;    Mesenchymal stem cell;   

DOI  :  10.1007/s00586-018-5839-y

来源: Springer

【 摘 要 】

Bone marrow aspirate has been successfully used alongside a variety of grafting materials to clinically augment spinal fusion. However, little is known about the fate of these transplanted cells. Herein, we develop a novel murine model for the in vivo monitoring of implanted bone marrow cells (BMCs) following spinal fusion. A clinical-grade scaffold was implanted into immune-intact mice undergoing spinal fusion with or without freshly isolated BMCs from either transgenic mice which constitutively express the firefly luciferase gene or syngeneic controls. The in vivo survival, distribution and proliferation of these luciferase-expressing cells was monitored via bioluminescence imaging over a period of 8 weeks and confirmed via immunohistochemistry. MicroCT imaging was performed 8 weeks to assess fusion. Bioluminescence imaging indicated transplanted cell survival and proliferation over the first 2 weeks, followed by a decrease in cell numbers, with transplanted cell survival still evident at the end of the study. New bone formation and increased fusion mass volume were observed in mice implanted with cell-seeded scaffolds. By enabling the tracking of transplanted bone marrow-derived cells during spinal fusion in vivo, this mouse model will be integral to developing a deeper understanding of the biological processes underlying spinal fusion in future studies. These slides can be retrieved under Electronic Supplementary Material.

【 授权许可】

Unknown