Bioinformatics analysis of calcium-dependent protein kinase 4 (CDPK4) as Toxoplasma gondii vaccine target

BMC Research Notes

Mohamad Sabaghan¹  Ali Dalir Ghaffari²  Ali Taghipour²  Masoud Foroutan³  Shahrzad Soltani³  Hamidreza Majidiani⁴ 

[1] Behbahan Faculty of Medical Sciences, Behbahan, Iran;Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, P. O. Box 14115-111, Tehran, Iran;USERN Office, Abadan Faculty of Medical Sciences, Abadan, Iran;Zoonotic Diseases Research Center, Ilam University of Medical Sciences, Ilam, Iran;

关键词: Toxoplasma gondii;    Calcium-dependent protein kinase 4;    Bioinformatics analysis;    In silico;    Vaccine;   

DOI  :  10.1186/s13104-021-05467-1

来源: Springer

【 摘 要 】

ObjectivesToxoplasma gondii (T. gondii), an obligate intracellular apicomplexan parasite, could affect numerous warm-blooded animals, such as humans. Calcium-dependent protein kinases (CDPKs) are essential Ca2+ signaling mediators and participate in parasite host cell egress, outer membrane motility, invasion, and cell division.ResultsSeveral bioinformatics online servers were employed to analyze and predict the important properties of CDPK4 protein. The findings revealed that CDPK4 peptide has 1158 amino acid residues with average molecular weight (MW) of 126.331 KDa. The aliphatic index and GRAVY for this protein were estimated at 66.82 and – 0.650, respectively. The findings revealed that the CDPK4 protein comprised 30.14% and 34.97% alpha-helix, 59.84% and 53.54% random coils, and 10.02% and 11.49% extended strand with SOPMA and GOR4 tools, respectively. Ramachandran plot output showed 87.87%, 8.40%, and 3.73% of amino acid residues in the favored, allowed, and outlier regions, respectively. Also, several potential B and T-cell epitopes were predicted for CDPK4 protein through different bioinformatics tools. Also, antigenicity and allergenicity evaluation demonstrated that this protein has immunogenic and non-allergenic nature. This paper presents a basis for further studies, thereby provides a fundamental basis for the development of an effective vaccine against T. gondii infection.

【 授权许可】

CC BY