In silico Analysis of Immunologic Regions of Surface Antigens (Sags) of Toxoplasma gondii

Shahid Beheshti University of Medical Sciences

Mojgan Bandehpour¹  Bahram Kazemi¹  Abbas Alibakhshi²  Tina Nafarieh²  Shivasadat Gheflat² 

[1] Department of Biotechnology, School of Advanced Technologies in Medicine,and Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran;Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

关键词: Epitope;    Agretope;    SAG;    Toxoplasma gondii;    In silico;   

DOI  :  10.22037/nbm.v5i3.14167

学科分类：生物科学（综合）

来源: Shahid Beheshti University of Medical Sciences

【 摘 要 】

Background: Surface antigens (SAGs) of Toxoplasma gondii are known candidates for diagnostic tests and vaccines. The present study argues about the main necessary properties for determination and prediction of T-cell agretopes and B-cell epitopes of surface antigens of Toxoplasma gondii. Materials and Methods:Primary, secondary and tertiary structures of the proteins were analyzed by different methods. The three-dimensional structures were determined by use ofab initiomethod for prediction of discontinues epitopes. The agretopes and epitopes were predicted via several various web servers with different methods employed. Results:The results ofin silicoanalyses showed that the regions 129-GAPAGRNNDGSSAPT-143 for protein p22, 234-SENPWQGNASSD-245 for protein p30 and 348-PGTEGESQAGT-358 for protein p43, have the highest immunogenic potential. Conclusion:We reached to three antigenic epitopes for cloning and protein expression. In following the purified polypeptide will be applied for diagnosis of Toxoplasma gondii.

【 授权许可】

CC BY