Regulation of Mg2+ uptake in isolated rat myocytes and hepatocytes by protein kinase C

FEBS Letters

Scarpa, A.¹  Romani, A.¹  Marfella, C.¹ 

[1] Department of Physiology and Biophysics, Case Western Reserve University, School of Medicine, Cleveland, OH 44106, USA

关键词: Mg2+ transport;    Protein kinase C;    Hepatocyte;    Myocyte;    HEPES;    4-(2-hydroxymethyl)-1 piperazine ethane sulphonic acid;    PIPES;    piperazine-N-N′-bis[2-ethane-sulfonic acid];    EGTA;    ethylene glycol-bis(b-aminoethyl ether) N;    N;    N′;    N′;    -tetraacetic acid;    EDTA;    ethylenediamine-tetraacetic acid;    FCCP;    carbonyl-cyanide p-(trifluoromethoxy-)phenylhydrazone;    AA;    atomic absorbance spectroscopy;    OAG;    1-oleoyl-2-acetyl-sn-glycerol (C18:1;    [cis]-9/ C2:0);    PKC;    protein kinase C;    SAG;    1-stearoyl-2-arachidonoyl-sn-glycerol (C18:0/C20:4;    [cis;    cis;    cis;    cis]-5;    8;    11;    14);    PDBU;    phorbol 12;    13-dibutyrate;    TPA;    phorbol 12-myristate 13-acetate;    H7;    1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride;   

DOI  :  10.1016/0014-5793(92)80364-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A large Mg²⁺ cell uptake against concentration gradients is stimulated in collagenase-dispersed rut myocytes by carbachol and in hepatocytes by carbachol or vasopressin. The signalling pathway(s) responsible for this stimulation of Mg²⁺ uptake was investigated by using various activators or inhibitors of protein kinase C (PKC) and by correlating Mg²⁺ uptake with cell PKC activity and cAMP content. In both cell preparations, the direct stimulation or PKC by diacylglycerol analogs or phorbol esters reproduce the same pattern of Mg²⁺ uptake as that induced by carbachol or vasopressin, These data indicate that the activation of PKC is responsible for a stimulation of Mg²⁺ uptake by myocytes or hepatocytes, whereas increase in cAMP in these cells stimulates Mg²⁺ release.

【 授权许可】

Unknown