Turkish Journal of Pharmaceutical Sciences | |
Simultaneous Determination of Hydrochlorothiazide and Irbesartan from Pharmaceutical Dosage Forms with RP-HPLC | |
article | |
Sevinç KURBANOĞLU1  Aysu YARMAN2  | |
[1] Ankara University Faculty of Pharmacy, Department of Analytical Chemistry;University of Potsdam, Institute of Biochemistry and Biology | |
关键词: HPLC; irbesartan; hydrochlorothiazide; pharmaceutical dosage forms; | |
DOI : 10.4274/tjps.galenos.2019.76094 | |
来源: Galenos Yayinevi | |
【 摘 要 】
Objectives: In this work, a simple and rapid liquid chromatographic method for the simultaneous determination of irbesartan (IRBE) and hydrochlorothiazide (HCT) was developed and validated by reverse phase high performance liquid chromatography (RP-HPLC). Materials and Methods: Experimental conditions such as different buffer solutions, various pH values, temperature, composition of the mobile phase, and the effect of flow rate were optimized. Results: The developed RP-HPLC method for these antihypertensive agents was wholly validated and IRBE was detected in the linear range of 0.1-25 μg mL-1 and HCT was detected in the linear range of 0.25-25 μg mL-1. Moreover, the suggested chromatographic technique was successfully applied for the determination of the drugs in human serum and pharmaceutical dosage forms with limit of detection values of 0.008 µg mL-1 for IRBE and 0.012 µg mL-1 for HCT. Conclusion: The proposed rapid analysis method of these antihypertensive drugs can be easily used and applied by pharmaceutical companies for which the analysis time is important.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO202106150000480ZK.pdf | 305KB | download |