Determination of Zn2+ in Solid Pharmaceutical Dosage Forms by Means of Spectrophotometry in Micellar Media: Method Validation

Applied Sciences

Simona-Codruța Hegheș¹  Luca Liviu Rus²  Anca Butuca²  Veronica Isabela Crăciun²  Andreea Loredana Vonica-Ţincu²  Claudiu Morgovan²  Anca Maria Juncan²  Andrei Catalin Muntean²  Carmen Maximiliana Dobrea²  Adriana Aurelia Chis²  Adina Frum² 

[1] Department of Drug Analysis, “Iuliu Hațieganu” University of Medicine and Pharmacy, 6 Pasteur Street, 400349 Cluj-Napoca, Romania;Preclinical Department, Faculty of Medicine, “Lucian Blaga” University, 2A, Lucian Blaga Street, 550169 Sibiu, Romania;

关键词: zinc;    spectrophotometry;    pharmaceutical dosage forms;    analytical method validation;    AAS method;   

DOI  :  10.3390/app12094476

来源: DOAJ

【 摘 要 】

In this study, a spectrophotometric method for the quantitative determination of zinc in a dietary supplement was validated. Zinc forms a ternary complex with Xylenol Orange and cetylpyridinium chloride in acetate buffer at pH = 5.5. The complex exhibits an absorption maximum at 560 nm. Linearity (range was 0.5–1.5 µg/mL, and correlation coefficient of the calibration curve was 0.9998), precision (repeatability variation coefficient and reproducibility variation coefficient were 1.57% and 1.77%, respectively), and accuracy (confidence interval of the mean recovery was 98.29–100.55%) of this method fulfil current pharmacopeial requests. Results obtained by spectrophotometric method were statistically compared with ones obtained by atomic absorption spectrometry and good agreement was found. The method can be applied routinely for quality control of solid pharmaceutical dosage forms containing zinc.

【 授权许可】

Unknown