The lysosomal membrane protein LAMP-2 is dispensable for PINK1/Parkin-mediated mitophagy

FEBS Letters

article

Xuemei Liu¹  Xinyi Liao²  Xiyun Rao²  Bin Wang²  Jun Zhang²  Ge Xu²  Xuejun Jiang³  Xia Qin⁴  Chengzhi Chen¹  Zhen Zou² 

[1] Department of Occupational and Environmental Health, Chongqing Medical University;Institute of Life Sciences, Chongqing Medical University;Center of Experimental Teaching for Public Health, Experimental Teaching and Management Center, Chongqing Medical University;Department of Pharmacy, The First Affiliated Hospital of Chongqing Medical University;Dongsheng Lung-Brain Disease Joint Lab, Chongqing Medical University

关键词: LAMP-2;    mitochondria;    mitophagy;    Parkin;    PINK1;   

DOI  :  10.1002/1873-3468.13663

来源: John Wiley & Sons Ltd.

【 摘 要 】

Selective autophagy for the elimination of aberrant mitochondria, termed mitophagy, can be regulated by the kinase PINK1 and the ubiquitin ligase Parkin. The lysosome-associated membrane protein 2 (LAMP-2) plays diverse functions in non-selective autophagy, chaperone-mediated autophagy and selective autophagy for the degradation of RNA/DNA. In the present study, we investigated whether LAMP-2 plays important roles during PINK1/Parkin-mediated mitophagy. The results obtained clearly show that knockdown of LAMP-2 does not cause defects in mitophagy in HeLa cells stably expressing Parkin, indicating that LAMP-2 is dispensable for PINK1/ Parkin-mediated mitophagy. The present study is the first to determine the potential role of LAMP-2 in PINK1/Parkin-mediated mitophagy, thereby providing more insight into the sophisticated process of mitophagy.

【 授权许可】

Unknown