FEBS Letters | |
MiR-21-5p regulates mycobacterial survival and inflammatory responses by targeting Bcl-2 and TLR4 in Mycobacteriumtuberculosis -infected macrophages | |
article | |
Zhonghua Zhao1  Jinzhu Hao1  Xia Li1  Yanfang Chen1  Xiaoyan Qi1  | |
[1] Department of Tuberculosis, Shandong Provincial Chest Hospital | |
关键词: Bcl-2; macrophages; miR-21-5p; Mycobacterium tuberculosis; survival; TLR4; | |
DOI : 10.1002/1873-3468.13438 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
To date, very little is known about the role of microRNA-21-5p (miR-21-5p) in Mycobacterium tuberculosis (M.tb)-infected macrophages. Here, we show that M.tb infection of RAW264.7 and THP-1 cells increases the expression of miR-21-5p. MiR-21-5p enhances M.tb survival and apoptosis, and attenuates the secretion of inflammatory cytokines, including interleukin (IL)-1b, IL-6, and tumor necrosis factor-a in M.tb-infected macrophages. Dualluciferase reporter assay revealed that the 30 -UTR of B-cell lymphoma 2 (Bcl2) or toll-like receptor 4 (TLR4) is a direct target of miR-21-5p. Enforced expressions of Bcl-2 or TLR4 partially attenuate the suppressive effects of miR-21-5p on cell viability and inflammatory cytokines, and effectively decrease bacterial burden. Therefore, the present study highlights a novel role for miR-21-5p in regulation of mycobacterial survival and inflammatory responses by targeting Bcl-2 and TLR4 in M.tb-infected macrophages.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO202105310000152ZK.pdf | 1980KB | download |