The genetic requirements of fatty acid import by Mycobacterium tuberculosis within macrophages

eLife

Brian C VanderVen¹  Thuy La¹  Evgeniya V Nazarova¹  Christine R Montague¹  David Russell¹  Lu Huang¹ 

[1] Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, United States;

关键词: Mycobacterium tuberculosis;    tuberculosis;    macrophages;    Mce1;    fatty acids;    LucA;   

DOI  :  10.7554/eLife.43621

来源: DOAJ

【 摘 要 】

Mycobacterium tuberculosis (Mtb) imports and metabolizes fatty acids to maintain infection within human macrophages. Although this is a well-established paradigm, the bacterial factors required for fatty acid import are poorly understood. Previously, we found that LucA and Mce1 are required for fatty acid import in Mtb (Nazarova et al., 2017). Here, we identified additional Mtb mutants that have a reduced ability to import a fluorescent fatty acid substrate during infection within macrophages. This screen identified the novel genes as rv2799 and rv0966c as be necessary for fatty acid import and confirmed the central role for Rv3723/LucA and putative components of the Mce1 fatty acid transporter (Rv0200/OmamB, Rv0172/Mce1D, and Rv0655/MceG) in this process.

【 授权许可】

Unknown