Antimicrobial Activity of 11 Insects Extracts Against Multi Drug Resistant (MDR) Strains of Bacteria and Fungus

2018 4th International Conference on Environmental Science and Material Application

生态环境科学;材料科学

Ma, Guangqiang^1 ; Wu, Lei^2 ; Shao, Feng^1 ; Zhang, Changhua^1 ; H., Wan

Jiangxi University of Traditional Chinese Medicine, Nanchang, China^1

Institute of Applied Chemistry, Jiangxi Academy of Science., Nanchang, China^2

关键词: Anti-bacterial activity;    Anti-microbial activity;    Disk diffusion tests;    Extraction solvents;    Minimum inhibitory concentration;    Mycobacterium tuberculosis;    Staphylococcus aureus;    Traditional Chinese Medicine;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/252/2/022132/pdf
DOI  :  10.1088/1755-1315/252/2/022132

来源: IOP

【 摘 要 】

Objective: 11 kinds of insects, which are traditional Chinese medicine (TCM), were detected by antimicrobial experiments to find new natural antibacterial extractives. Method: The body or shell of insects were extracted by two stage extraction solvent. Firstly, the body were impregnated two times with dichloromethane solvent, and then, soaked with 70% methanol and extracted two times. Disk diffusion test and Broth Dilution Assay were used to test MIC (MIC-minimum inhibitory concentration) value of these extractions. Multi Drug Resistant (MDR) Strains of Bacteria and Fungus were used as detected strains in the test. Result: Of all eleven species of insects, extractives of the shell of Cryptotympanapustulata Fabricius and the body of Mole cricket have significant antibacterial activity on Staphylococcus aureus and Mycobacterium tuberculosis; the different organic solvents components of C. Fabricius and M.cricket also have inhibit effects on S.aureus and M.tuberculosis; The MIC of the ethyl acetate fraction of M.cricket on M.tuberculosis was even reached to 0.01 g/mL. Moreover, the effect of water extractives from C. Fabricius on M.tuberculosis was 0.01 g/mL eighter. Discussion: The discovery of new antimicrobial medicine is always an urgent and successive need in the field of Medicine. Insects is a new field for the discovery of antibiotics.