Understanding seasonal weight loss tolerance in dairy goats: a transcriptomics approach

BMC Genomics

Anastasio Argüello¹  Noemí Castro¹  Lorenzo Enrique Hernández-Castellano²  José Ricardo Parreira³  Susana de Sousa Araújo⁴  André Martinho de Almeida⁵  Juan Capote⁶ 

[1] Animal Production and Biotechnology group, Institute of Animal Health and Food Safety, Universidad de Las Palmas de Gran Canaria, 35413, Arucas, Spain;Department of Animal Science, AU-Foulum, Aarhus University, 8830, Tjele, Denmark;IBET – Instituto de Biologia Experimental e Tecnológica, Av. da República, 2780-157, Oeiras, Portugal;ITQB NOVA – Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157, Oeiras, Portugal;ITQB NOVA – Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157, Oeiras, Portugal;LEAF - Linking Landscape, Environment, Agriculture And Food, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 13409-017, Lisbon, Portugal;Unit of Animal Production, Pasture, and Forage in Arid and Subtropical Areas, Canary Islands Institute for Agricultural Research, 38270, La Laguna, Spain;

关键词: Goat;    Mammary gland;    Transcriptomics;    RNA-sequencing;    Seasonal weight loss;   

DOI  :  10.1186/s12864-020-06968-2

来源: Springer

【 摘 要 】

BackgroundSeasonal weight loss (SWL) is a very important limitation to the production of ruminants in the Mediterranean and Tropical regions. In these areas, long dry seasons lead to poor pastures with low nutritional value. During the dry season, ruminants, particularly those raised in extensive production systems, lose around 30% of their body weight. Seasonal weight loss has important consequences on animal productive performance and health. In this study, RNA sequencing was used to characterize feed restriction effects in dairy goat of 2 breeds with different SWL tolerance: Majorera (tolerant) and Palmera (susceptible). Nine Majorera and ten Palmera goats were randomly distributed in a control and a restricted group: Majorera Control (adequately fed; MC; n = 4), Palmera Control (adequately fed; PC; n = 6), Majorera Restricted (feed restricted; ME; n = 5) and Palmera Restricted (feed restricted; PE; n = 4). On day 22 of the trial, mammary gland biopsies were collected for transcriptomics analysis.ResultsFrom these samples, 24,260 unique transcripts were identified. From those, 82 transcripts were differentially expressed between MC and ME, 99 between PC and PE, twelve between both control groups and twenty-nine between both restricted groups.ConclusionsFeed restriction affected several biochemical pathways in both breeds such as: carbohydrate and lipid transport; intracellular trafficking, RNA processing and signal transduction. This research also highlights the importance or involvement of the genes in tolerance (ENPP1, S-LZ, MT2A and GPNB) and susceptibility (GPD1, CTPS1, ELOVL6 and NR4A1) to SWL with respectively higher expression in the Majorera restriced group and the Palmera restricted group in comparison to the control groups. In addition, results from the study may be extrapolated to other dairy ruminant species.

【 授权许可】

CC BY