Transcriptome analysis reveals liver metabolism programming in kids from nutritional restricted goats during mid-gestation

PeerJ

article

Chao Yang¹  Xiaoling Zhou¹  Hong Yang¹  Kefyalew Gebeyew¹  Qiongxian Yan¹  Chuanshe Zhou¹  Zhixiong He¹  Zhiliang Tan¹ 

[1] CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South-Central Experimental Station of Animal Nutrition and Feed Science, Institute of Subtropical Agriculture, The Chinese Academy of Sciences;University of Chinese Academy of Sciences;College of Animal Science, Tarim University;Hunan Co-Innovation Center of Animal Production Safety

关键词: Goat;    Maternal undernutrition;    Hepatic metabolism;    RNA-sequencing;   

DOI  :  10.7717/peerj.10593

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundMaternal nutrient restriction during pregnancy causes a metabolic disorder that threatens the offspring’s health in humans and animals. However, the molecular mechanism of how undernutrition affecting hepatic metabolism of fetal or postnatal offspring is still unclear. We aimed to investigate transcriptomic changes of fetal livers in response to maternal malnutrition in goats during mid-gestation and to explore whether these changes would disappear when the nutrition was recovered to normal level during mid-gestation using goats (Capra hircus) as the experimental animals.MethodsFifty-three pregnant goats were subjected to a control (100% of the maintenance requirements, CON) or a restricted (60% of the maintenance requirements on day 45 to day 100 of gestation and then realimentation, RES) diet. A total of 16 liver samples were collected from fetal goats on day 100 of gestation and goat kids of postnatal day 90 to obtain hepatic transcriptional profiles using RNA-Seq.Results1.2 and FDR < 0.1) within KR vs. KC comparison was identified with 79 up-regulated genes and down-regulated 39 genes, and these DEGs mainly enriched in the biosynthesis of amino acids, citrate cycle, valine, leucine and isoleucine biosynthesis and carbon metabolism.ConclusionHepatic transcriptome analysis showed that maternal undernutrition promoted protein digestion and absorption in the fetal livers, while which restrained carbohydrate metabolism and citric acid cycle in the livers of kid goats after realimentation. The results indicate that maternal undernutrition during mid-gestation causes hepatic metabolism programming in kid goats on a molecular level.

【 授权许可】

CC BY