Adverse effects in the fish embryo acute toxicity (FET) test: a catalogue of unspecific morphological changes versus more specific effects in zebrafish (Danio rerio) embryos

Environmental Sciences Europe

Rebecca von Hellfeld¹  Katharina Brotzmann¹  Thomas Braunbeck¹  Lisa Baumann¹  Ruben Strecker² 

[1] Aquatic Ecology and Toxicology, Centre for Organismal Studies, University of Heidelberg, Im Neuenheimer Feld 504, 69120, Heidelberg, Germany;Aquatic Ecology and Toxicology, Centre for Organismal Studies, University of Heidelberg, Im Neuenheimer Feld 504, 69120, Heidelberg, Germany;SCC GmbH, Am Grenzgraben 11, 55545, Bad Kreuznach, Germany;

关键词: Zebrafish;    OECD TG 236;    Embryo toxicity;    FET test;    Teratogenicity;    Spinal defects;    Lordosis;    Kyphosis;    Scoliosis;    Craniofacial deformation;    Yolk malabsorption;   

DOI  :  10.1186/s12302-020-00398-3

来源: Springer

【 摘 要 】

BackgroundThe Fish Embryo Acute Toxicity (FET) test with the zebrafish (Danio rerio) embryo, the OECD test guideline (TG) 236, has been designed as an alternative for acute fish toxicity testing such as the OECD Acute Fish Toxicity Test (TG 203). To provide equivalent sensitivity to the acute fish test, the original FET test was designed to use only four morphological core endpoints: coagulation of the embryo, lack of somite formation, lack of heart beat, and non-detachment of the tail. These endpoints were selected due to (1) their association with mortality, directly or indirectly, (2) improve the practicality for screening by well-trained technical staff, and (3) the endpoints being relatively simple morphological alterations.ResultsWith the growing need to understand the developmental toxicity of compounds found in the environment, the FET protocol has repeatedly been extended to a multitude of additional morphological endpoints that also allow the monitoring of teratogenicity. As the extensive use of the FET test has generated a multitude of observations in the scientific literature, a harmonisation of the terminology used for the description of the morphological effects seen after chemical exposure has become necessary.ConclusionFor this end, the present communication provides an overview of both common and selected more specific morphological effects seen in zebrafish embryos after exposure to a wide variety of chemical substances together with suggestions for a harmonised nomenclature.

【 授权许可】

CC BY