Development of an In Vitro System Detecting Pro-embryotoxin

The Japanese Journal of Pharmacology

Masaaki Miyata¹  Yasushi Yamazoe¹  Etsuko Tamura¹ 

[1] Division of Drug Metabolism and Molecular Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University

关键词: Embryo fibroblast;    Embryo toxicity;    Pre-incubation;   

DOI  :  10.1254/jjp.89.320

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(12)Cited-By(2)An in vitro system for detection of embryotoxins has been developed by using primary cultures of embryo fibroblasts. Various embryotoxins, including benzo[a]pyrene and thalidomide, have trivial cytotoxicity in embryo fibroblast systems, which is at least in part due to a lack of capacity for metabolic activation. Introduction of steps for microsomal pre-incubation and calcium-precipitation prior to chemical contact resulted in the clear appearance of embryotoxicity toward thalidomide and benzo[a]pyrene. This pre-incubation method will offer advantages for the detection of embryotoxins, which require maternal metabolic activation, and for understanding the mechanisms of their metabolic activations.

【 授权许可】

Unknown